PREY

LCK

Hck-3, Lsk, Lskt, p56, p56Lck, RP23-209C6.8
lymphocyte protein tyrosine kinase
GO Process (27)
GO Function (17)
GO Component (11)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Direct interaction of the CD38 cytoplasmic tail and the Lck SH2 domain. Cd38 transduces T cell activation signals through associated Lck.

Cho YS, Han MK, Choi YB, Yun Y, Shin J, Kim UH

CD38 ligation has been shown to induce activation of intracellular signaling cascade in T lymphocytes through a Lck-dependent pathway. However, it is not clear how Lck initiates the CD38-mediated signaling process. In the present study, we showed that CD38 and Lck were physically associated through the cytoplasmic tail and the Src homology 2 domain, respectively. This was evidenced by coimmunoprecipitation ... [more]

J. Biol. Chem. Jan. 21, 2000; 275(3);1685-90 [Pubmed: 10636863]

Throughput

  • Low Throughput

Curated By

  • BioGRID