BAIT
C15ORF59
chromosome 15 open reading frame 59
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY
CEBPG
GPE1BP, IG/EBP-1
CCAAT/enhancer binding protein (C/EBP), gamma
GO Process (12)
GO Function (6)
GO Component (2)
Gene Ontology Biological Process
- B cell differentiation [ISS]
- enucleate erythrocyte differentiation [ISS]
- immune response [ISS]
- liver development [ISS]
- natural killer cell mediated cytotoxicity [ISS]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISS]
- positive regulation of DNA binding [TAS]
- positive regulation of DNA repair [IEP]
- positive regulation of interferon-gamma biosynthetic process [ISS]
- positive regulation of sequence-specific DNA binding transcription factor activity [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding [IDA]
- transcription factor binding [IDA]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding [IDA]
- transcription factor binding [IDA]
Gene Ontology Cellular Component
- nucleoplasm [IDA]
- nucleus [ISS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.829626965 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.829626965, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID