BAIT

EIF2AK2

EIF2AK1, PKR, PPP1R83, PRKR
eukaryotic translation initiation factor 2-alpha kinase 2
GO Process (22)
GO Function (7)
GO Component (4)
Homo sapiens
PREY

STAT1

CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91
signal transducer and activator of transcription 1, 91kDa
GO Process (32)
GO Function (10)
GO Component (8)

Gene Ontology Biological Process

Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Enhanced antiviral and antiproliferative properties of a STAT1 mutant unable to interact with the protein kinase PKR.

Wong AH, Durbin JE, Li S, Dever TE, Decker T, Koromilas AE

We have previously reported a physical association between STAT1 and the protein kinase double-stranded RNA-activated protein kinase (PKR). PKR inhibited STAT1 function in a manner independent of PKR kinase activity. In this report, we have further characterized the properties of both molecules by mapping the sites of their interaction. A STAT1 mutant unable to interact with PKR displays enhanced interferon ... [more]

J. Biol. Chem. Apr. 27, 2001; 276(17);13727-37 [Pubmed: 11278865]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
STAT1 EIF2AK2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
EIF2AK2 STAT1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID