Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

ERBIN associates with p0071, an armadillo protein, at cell-cell junctions of epithelial cells.

Izawa I, Nishizawa M, Tomono Y, Ohtakara K, Takahashi T, Inagaki M

BACKGROUND: ERBIN, an ErbB2 receptor-interacting protein, belongs to a recently described family of proteins termed the LAP [leucine-rich repeats and PSD-95/dLg-A/ZO-1 (PDZ) domains] family which has essential roles in establishment of cell polarity. RESULTS: To identify new ERBIN-binding proteins, we screened a yeast two-hybrid library, using the carboxyl-terminal fragment of ERBIN containing PDZ domain as the bait, and we isolated ... [more]

Genes Cells May. 01, 2002; 7(5);475-85 [Pubmed: 12047349]

Throughput

  • Low Throughput

Curated By

  • BioGRID