Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Different roles for the cyclic nucleotide binding domain and amino terminus in assembly and expression of hyperpolarization-activated, cyclic nucleotide-gated channels.

Proenza C, Tran N, Angoli D, Zahynacz K, Balcar P, Accili EA

In mammalian heart and brain, pacemaker currents are produced by hyperpolarization-activated, cyclic nucleotide-gated (HCN) channels, which probably exist as heteromeric assemblies of different subunit isoforms. To investigate the molecular domains that participate in assembly and membrane trafficking of HCN channels, we have used the yeast two-hybrid system, patch clamp electrophysiology, and confocal microscopy. We show here that the N termini ... [more]

J. Biol. Chem. Aug. 16, 2002; 277(33);29634-42 [Pubmed: 12034718]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

HCN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

cAMP binding [ISS]intracellular cAMP activated cation channel activity [ISS]potassium channel activity [NAS]voltage-gated potassium channel activity [IBA, IMP, ISS]voltage-gated sodium channel activity [IMP]

Gene Ontology Cellular Component