BAIT
PTPN22
LYP, LYP1, LYP2, PEP, PTPN8
protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
GO Process (9)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- T cell differentiation [ISS]
- negative regulation of T cell activation [IMP]
- negative regulation of T cell receptor signaling pathway [IDA]
- peptidyl-tyrosine dephosphorylation [IDA]
- phosphoanandamide dephosphorylation [ISS]
- protein dephosphorylation [IDA, TAS]
- regulation of B cell receptor signaling pathway [NAS]
- regulation of innate immune response [IC]
- regulation of natural killer cell proliferation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PRKAR2B
PRKAR2, RII-BETA
protein kinase, cAMP-dependent, regulatory, type II, beta
GO Process (18)
GO Function (4)
GO Component (7)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- activation of phospholipase C activity [TAS]
- activation of protein kinase A activity [TAS]
- blood coagulation [TAS]
- cellular response to glucagon stimulus [TAS]
- energy reserve metabolic process [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- intracellular signal transduction [TAS]
- mitotic cell cycle [TAS]
- negative regulation of cAMP-dependent protein kinase activity [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- regulation of insulin secretion [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
- water transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.972339874 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.972339874, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID