BAIT

PDLIM5

ENH, ENH1, LIM, L9
PDZ and LIM domain 5
GO Process (2)
GO Function (3)
GO Component (4)
Homo sapiens
PREY

PRKCE

PKCE, nPKC-epsilon
protein kinase C, epsilon
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Protein-protein interaction of zinc finger LIM domains with protein kinase C.

Kuroda S, Tokunaga C, Kiyohara Y, Higuchi O, Konishi H, Mizuno K, Gill GN, Kikkawa U

The LIM domain comprising two zinc-finger motifs is found in a variety of proteins and has been proposed to direct protein-protein interactions. During the identification of protein kinase C (PKC)-interacting proteins by a yeast two-hybrid assay, a novel protein containing three LIM domains, designated ENH, was shown to associate with PKC in an isoform-specific manner. Deletion analysis demonstrated that any ... [more]

J. Biol. Chem. Dec. 06, 1996; 271(49);31029-32 [Pubmed: 8940095]

Throughput

  • Low Throughput

Curated By

  • BioGRID