BAIT

KHDRBS3

Etle, Slm2
KH domain containing, RNA binding, signal transduction associated 3
GO Process (2)
GO Function (4)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Rattus norvegicus
PREY

HNRNPL

HNRPL, hnRNP-L, P/OKcl.14
heterogeneous nuclear ribonucleoprotein L
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The STAR/GSG family protein rSLM-2 regulates the selection of alternative splice sites.

Stoss O, Olbrich M, Hartmann AM, Konig H, Memmott J, Andreadis A, Stamm S

We identified the rat Sam68-like mammalian protein (rSLM-2), a member of the STAR (signal transduction and activation of RNA) protein family as a novel splicing regulatory protein. Using the yeast two-hybrid system, coimmunoprecipitations, and pull-down assays, we demonstrate that rSLM-2 interacts with various proteins involved in the regulation of alternative splicing, among them the serine/arginine-rich protein SRp30c, the splicing-associated factor ... [more]

J. Biol. Chem. Mar. 23, 2001; 276(12);8665-73 [Pubmed: 11118435]

Throughput

  • Low Throughput

Curated By

  • BioGRID