PREY

PAFAH1B1

LIS-1, Lis1, MMS10-U, Mdsh, Ms10u, Pafaha, RP23-194P5.2
platelet-activating factor acetylhydrolase, isoform 1b, subunit 1
GO Process (35)
GO Function (7)
GO Component (22)
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome.

Feng Y, Olson EC, Stukenberg PT, Flanagan LA, Kirschner MW, Walsh CA

LIS1, a microtubule-associated protein, is required for neuronal migration, but the precise mechanism of LIS1 function is unknown. We identified a LIS1 interacting protein encoded by a mouse homolog of NUDE, a nuclear distribution gene in A. nidulans and a multicopy suppressor of the LIS1 homolog, NUDF. mNudE is located in the centrosome or microtubule organizing center (MTOC), and interacts ... [more]

Neuron Dec. 01, 2000; 28(3);665-79 [Pubmed: 11163258]

Throughput

  • Low Throughput

Curated By

  • BioGRID