BAIT

PRKCZ

14-3-3-zetaisoform, Pkcz, r14-3-3
protein kinase C, zeta
GO Process (43)
GO Function (10)
GO Component (21)

Gene Ontology Biological Process

Rattus norvegicus
PREY

KCNAB2

potassium voltage-gated channel, shaker-related subfamily, beta member 2
GO Process (2)
GO Function (1)
GO Component (2)
Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Differential stimulation of PKC phosphorylation of potassium channels by ZIP1 and ZIP2.

Gong J, Xu J, Bezanilla M, van Huizen R, Derin R, Li M

Targeting of protein modification enzymes is a key biochemical step to achieve specific and effective posttranslational modifications. Two alternatively spliced ZIP1 and ZIP2 proteins are described, which bind to both Kvbeta2 subunits of potassium channel and protein kinase C (PKC) zeta, thereby acting as a physical link in the assembly of PKCzeta-ZIP-potassium channel complexes. ZIP1 and ZIP2 differentially stimulate phosphorylation ... [more]

Science Sep. 03, 1999; 285(5433);1565-9 [Pubmed: 10477520]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KCNAB2 PRKCZ
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID