SLIT2
Gene Ontology Biological Process
- Roundabout signaling pathway [IC, IMP]
- apoptotic process involved in luteolysis [IEP]
- axon extension involved in axon guidance [IDA]
- axon guidance [IDA, TAS]
- branching morphogenesis of an epithelial tube [IDA, IMP]
- cell migration involved in sprouting angiogenesis [IMP]
- cellular response to heparin [IDA]
- cellular response to hormone stimulus [IEP]
- chemorepulsion involved in embryonic olfactory bulb interneuron precursor migration [IDA]
- chemorepulsion involved in postnatal olfactory bulb interneuron migration [IDA]
- corticospinal neuron axon guidance through spinal cord [IMP]
- induction of negative chemotaxis [IDA]
- motor neuron axon guidance [IDA]
- negative chemotaxis [IDA, IMP]
- negative regulation of actin filament polymerization [IDA]
- negative regulation of catalytic activity [IDA]
- negative regulation of cell growth [IMP]
- negative regulation of cell migration [IDA, IMP]
- negative regulation of cellular response to growth factor stimulus [IDA]
- negative regulation of chemokine-mediated signaling pathway [IMP]
- negative regulation of endothelial cell migration [IDA]
- negative regulation of lamellipodium assembly [IDA]
- negative regulation of leukocyte chemotaxis [IDA]
- negative regulation of monocyte chemotaxis [ISS]
- negative regulation of mononuclear cell migration [IDA]
- negative regulation of neutrophil chemotaxis [IDA]
- negative regulation of protein phosphorylation [IDA]
- negative regulation of retinal ganglion cell axon guidance [IDA]
- negative regulation of small GTPase mediated signal transduction [IDA]
- negative regulation of smooth muscle cell chemotaxis [IDA]
- negative regulation of smooth muscle cell migration [IDA]
- negative regulation of vascular permeability [IDA]
- positive regulation of apoptotic process [IMP]
- positive regulation of axonogenesis [TAS]
- response to cortisol [IEP]
- retinal ganglion cell axon guidance [IDA]
- ureteric bud development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GPC1
Gene Ontology Biological Process
- Schwann cell differentiation [ISS]
- axon guidance [TAS]
- carbohydrate metabolic process [TAS]
- chondroitin sulfate metabolic process [TAS]
- glycosaminoglycan biosynthetic process [TAS]
- glycosaminoglycan catabolic process [TAS]
- glycosaminoglycan metabolic process [TAS]
- heparan sulfate proteoglycan catabolic process [IDA]
- myelin assembly [ISS]
- negative regulation of fibroblast growth factor receptor signaling pathway [ISS]
- phototransduction, visible light [TAS]
- positive regulation of skeletal muscle cell differentiation [ISS]
- retinoid metabolic process [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Characterization of Slit protein interactions with glypican-1.
We have demonstrated previously that the Slit proteins, which are involved in axonal guidance and related developmental processes in nervous tissue, are ligands of the glycosylphosphatidylinositol-anchored heparan sulfate proteoglycan glypican-1 in brain (Liang, Y., Annan, R. S., Carr, S. A., Popp, S., Mevissen, M., Margolis, R. K., and Margolis, R. U. (1999) J. Biol. Chem. 274, 17885--17892). To characterize these ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SLIT2 GPC1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID