BAIT

RRAD

RAD, RAD1, REM3
Ras-related associated with diabetes
Human Papilloma Virus (HPV) Project
GO Process (0)
GO Function (2)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens
PREY

TPM2

Tpm-2, Trop-2, RP23-195K8.10-003
tropomyosin 2, beta
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Rad, a novel Ras-related GTPase, interacts with skeletal muscle beta-tropomyosin.

Zhu J, Bilan PJ, Moyers JS, Antonetti DA, Kahn CR

Rad, a prototypic member of a subfamily of Ras-related GTPases, is overexpressed in skeletal muscle of type II diabetic humans. By expression screening of mouse embryo and human skeletal muscle cDNA libraries, we found that Rad interacted with skeletal muscle beta-tropomyosin. In the mouse skeletal muscle cell line C2C12, this interaction was significantly increased by the calcium ionophore A23187. A23187 ... [more]

J. Biol. Chem. Jan. 12, 1996; 271(2);768-73 [Pubmed: 8557685]

Throughput

  • Low Throughput

Curated By

  • BioGRID