BAIT

RRAD

REM3, Rad
Ras-related associated with diabetes
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

TPM2

Tpm-2, Trop-2, RP23-195K8.10-003
tropomyosin 2, beta
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Rad, a novel Ras-related GTPase, interacts with skeletal muscle beta-tropomyosin.

Zhu J, Bilan PJ, Moyers JS, Antonetti DA, Kahn CR

Rad, a prototypic member of a subfamily of Ras-related GTPases, is overexpressed in skeletal muscle of type II diabetic humans. By expression screening of mouse embryo and human skeletal muscle cDNA libraries, we found that Rad interacted with skeletal muscle beta-tropomyosin. In the mouse skeletal muscle cell line C2C12, this interaction was significantly increased by the calcium ionophore A23187. A23187 ... [more]

J. Biol. Chem. Jan. 12, 1996; 271(2);768-73 [Pubmed: 8557685]

Throughput

  • Low Throughput

Curated By

  • BioGRID