BAIT

RAPGEF6

KIA001LB, PDZ-GEF2, PDZGEF2, RA-GEF-2, RAGEF2
Rap guanine nucleotide exchange factor (GEF) 6
GO Process (3)
GO Function (4)
GO Component (1)
Homo sapiens
PREY

MRAS

M-RAs, R-RAS3, RRAS3
muscle RAS oncogene homolog
GO Process (4)
GO Function (2)
GO Component (1)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Identification and characterization of RA-GEF-2, a Rap guanine nucleotide exchange factor that serves as a downstream target of M-Ras.

Gao X, Satoh T, Liao Y, Song C, Hu CD, Kariya Ki K, Kataoka T

The Ras family small GTPase Rap is regulated by an array of specific guanine nucleotide exchange factors (GEFs) in response to upstream stimuli. RA-GEF-1 was identified as a novel Rap GEF, which possesses a Ras/Rap1-associating (RA) domain. Here we report a protein closely related to RA-GEF-1, named RA-GEF-2. Like RA-GEF-1, a putative cyclic nucleotide monophosphate-binding domain, a Ras exchanger motif, ... [more]

J. Biol. Chem. Nov. 09, 2001; 276(45);42219-25 [Pubmed: 11524421]

Throughput

  • Low Throughput

Curated By

  • BioGRID