An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Eps15 is constitutively oligomerized due to homophilic interaction of its coiled-coil region.

Tebar F, Confalonieri S, Carter RE, Di Fiore PP, Sorkin A

Eps15 is a member of an emerging family of proteins containing a novel protein/protein interaction domain, the EH domain, of as yet unknown function. Recent findings of Eps15 association with clathrin adaptor complex AP-2 and its localization in clathrin-coated pits have implicated Eps15 in the regulation of vesicle trafficking. Here we show that Eps15 exists in several multimeric states in ... [more]

J. Biol. Chem. Jun. 13, 1997; 272(24);15413-8 [Pubmed: 9182572]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
EPS15 EPS15	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Tebar F (1997)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

EPS15

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]polyubiquitin binding [ISO]protein binding [IPI]

Gene Ontology Cellular Component