BAIT

AP2B1

1300012O03Rik, AI788979, RP23-381B19.6
adaptor-related protein complex 2, beta 1 subunit
GO Process (0)
GO Function (2)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

EPS15

2410112D09Rik, RP23-414M10.3
epidermal growth factor receptor pathway substrate 15
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Eps15 is constitutively oligomerized due to homophilic interaction of its coiled-coil region.

Tebar F, Confalonieri S, Carter RE, Di Fiore PP, Sorkin A

Eps15 is a member of an emerging family of proteins containing a novel protein/protein interaction domain, the EH domain, of as yet unknown function. Recent findings of Eps15 association with clathrin adaptor complex AP-2 and its localization in clathrin-coated pits have implicated Eps15 in the regulation of vesicle trafficking. Here we show that Eps15 exists in several multimeric states in ... [more]

J. Biol. Chem. Jun. 13, 1997; 272(24);15413-8 [Pubmed: 9182572]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EPS15 AP2B1
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.882BioGRID
2677737

Curated By

  • BioGRID