BAIT
TKT
HEL107, TK, TKT1
transketolase
GO Process (7)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TKT
HEL107, TK, TKT1
transketolase
GO Process (7)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Aspartate 155 of human transketolase is essential for thiamine diphosphate-magnesium binding, and cofactor binding is required for dimer formation.
Active human transketolase is a homodimeric enzyme possessing two active sites, each with a non-covalently bound thiamine diphosphate and magnesium. Both subunits contribute residues at each site which are involved in cofactor binding and in catalysis. His-tagged transketolase, produced in E. coli, was similar to transketolase purified from human tissues with respect to Km apps for cofactor and substrates and ... [more]
Biochim. Biophys. Acta Sep. 05, 1997; 1341(2);165-72 [Pubmed: 9357955]
Throughput
- Low Throughput
Curated By
- BioGRID