An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Aspartate 155 of human transketolase is essential for thiamine diphosphate-magnesium binding, and cofactor binding is required for dimer formation.

Wang JJ, Martin PR, Singleton CK

Active human transketolase is a homodimeric enzyme possessing two active sites, each with a non-covalently bound thiamine diphosphate and magnesium. Both subunits contribute residues at each site which are involved in cofactor binding and in catalysis. His-tagged transketolase, produced in E. coli, was similar to transketolase purified from human tissues with respect to Km apps for cofactor and substrates and ... [more]

Biochim. Biophys. Acta Sep. 05, 1997; 1341(2);165-72 [Pubmed: 9357955]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TKT

Gene Ontology Biological Process

Gene Ontology Molecular Function

cofactor binding [IDA]protein homodimerization activity [IDA]transketolase activity [EXP, IDA]

Gene Ontology Cellular Component

TKT