BAIT
ATG4C
APG4-C, APG4C, AUTL1, AUTL3
autophagy related 4C, cysteine peptidase
GO Process (10)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
ALS2
ALS2CR6, ALSJ, IAHSP, PLSJ
amyotrophic lateral sclerosis 2 (juvenile)
GO Process (9)
GO Function (8)
GO Component (9)
Gene Ontology Biological Process
- endosome organization [IGI, NAS]
- neuron projection morphogenesis [IDA]
- positive regulation of Rab GTPase activity [IDA]
- positive regulation of Rac GTPase activity [IDA]
- positive regulation of Rac protein signal transduction [IC]
- positive regulation of Ran GTPase activity [NAS]
- positive regulation of protein kinase activity [IDA]
- positive regulation of protein serine/threonine kinase activity [IDA]
- regulation of endosome size [IEP]
Gene Ontology Molecular Function- Rab GTPase binding [IDA, NAS]
- Rab guanyl-nucleotide exchange factor activity [IDA]
- Rac guanyl-nucleotide exchange factor activity [IDA]
- Ran guanyl-nucleotide exchange factor activity [NAS]
- guanyl-nucleotide exchange factor activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- protein serine/threonine kinase activator activity [IDA]
- Rab GTPase binding [IDA, NAS]
- Rab guanyl-nucleotide exchange factor activity [IDA]
- Rac guanyl-nucleotide exchange factor activity [IDA]
- Ran guanyl-nucleotide exchange factor activity [NAS]
- guanyl-nucleotide exchange factor activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- protein serine/threonine kinase activator activity [IDA]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.98687789 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.98687789, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID