BAIT

ACTA1

ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, RP5-1068B5.2
actin, alpha 1, skeletal muscle
Homo sapiens
PREY

TMSB4Y

TB4Y
thymosin beta 4, Y-linked
GO Process (1)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Thymosin beta 10 and thymosin beta 4 are both actin monomer sequestering proteins.

Yu FX, Lin SC, Morrison-Bogorad M, Atkinson MA, Yin HL

The beta-thymosins are a family of related peptides. Recently, thymosin beta 4 was identified as a significant actin monomer sequestering protein in cells. To determine if other beta-thymosins also bind actin, and how they may participate in the regulation of actin polymerization, we expressed thymosin beta 4 and its major homolog, thymosin beta 10, in bacteria and characterized their interactions ... [more]

J. Biol. Chem. Jan. 05, 1993; 268(1);502-9 [Pubmed: 8416954]

Throughput

  • Low Throughput

Curated By

  • BioGRID