An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

LL5beta is a phosphatidylinositol (3,4,5)-trisphosphate sensor that can bind the cytoskeletal adaptor, gamma-filamin.

Paranavitane V, Coadwell WJ, Eguinoa A, Hawkins PT, Stephens L

We identified a potential phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P(3)) binding pleckstrin homology domain in the data bases and have cloned and expressed its full coding sequence (LL5beta). The protein bound PtdIns(3,4,5)P(3) selectively in vitro. Strikingly, a substantial proportion of LL5beta became associated with an unidentified intracellular vesicle population in the context of low PtdIns(3,4,5)P(3) levels produced by the addition of wortmannin or ... [more]

J. Biol. Chem. Jan. 10, 2003; 278(2);1328-35 [Pubmed: 12376540]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PHLDB2 FLNC	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Paranavitane V (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

PHLDB2

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

FLNC

Gene Ontology Biological Process