BAIT

DLG4

PSD95, SAP-90, SAP90
discs, large homolog 4 (Drosophila)
GO Process (18)
GO Function (11)
GO Component (21)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

N-terminal PDZ-binding domain in Kv1 potassium channels.

Eldstrom J, Doerksen KW, Steele DF, Fedida D

We have investigated the interactions of prototypical PDZ domains with both the C- and N-termini of Kv1.5 and other Kv channels. A combination of in vitro binding and yeast two-hybrid assays unexpectedly showed that PDZ domains derived from PSD95 bind both the C- and N-termini of the channels with comparable avidity. From doubly transfected HEK293 cells, Kv1.5 was found to ... [more]

FEBS Lett. Nov. 20, 2002; 531(3);529-37 [Pubmed: 12435606]

Throughput

  • Low Throughput

Curated By

  • BioGRID