An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Amorphin is phosphorylase; phosphorylase is an alpha-actinin-binding protein.

Chowrashi P, Mittal B, Sanger JM, Sanger JW

In a study of myofibrillar proteins, Chowrashi and Pepe [1982: J. Cell Biol. 94:565-573] reported the isolation of a new, 85-kD Z-band protein that they named amorphin. We report that partial sequences of purified amorphin protein indicate that amorphin is identical to phosphorylase, an enzyme important in the metabolism of glycogen. Anti-amorphin antibodies also reacted with purified chicken and rabbit ... [more]

Cell Motil. Cytoskeleton Oct. 01, 2002; 53(2);125-35 [Pubmed: 12211109]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ACTN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

double-stranded RNA binding [IDA]integrin binding [IDA]ion channel binding [IPI]protein binding [IPI]vinculin binding [IPI]

Gene Ontology Cellular Component

PYGB