BAIT
PHF8
JHDM1F, MRXSSD, ZNF422, RP13-444K19.2
PHD finger protein 8
GO Process (10)
GO Function (11)
GO Component (4)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IMP]
- brain development [ISS]
- histone H3-K27 demethylation [IDA]
- histone H3-K36 demethylation [IDA]
- histone H3-K9 demethylation [IDA]
- histone H4-K20 demethylation [IDA]
- mitotic cell cycle [TAS]
- negative regulation of chromatin silencing at rDNA [IDA]
- positive regulation of transcription from RNA polymerase I promoter [IDA]
- positive regulation of transcription, DNA-templated [IDA, IMP]
Gene Ontology Molecular Function- chromatin binding [IDA]
- histone demethylase activity [IDA]
- histone demethylase activity (H3-K27 specific) [IDA]
- histone demethylase activity (H3-K36 specific) [IDA]
- histone demethylase activity (H3-K9 specific) [IDA]
- histone demethylase activity (H4-K20 specific) [IDA]
- iron ion binding [IDA]
- methylated histone binding [IDA]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [IDA]
- protein binding [IPI]
- zinc ion binding [IDA]
- chromatin binding [IDA]
- histone demethylase activity [IDA]
- histone demethylase activity (H3-K27 specific) [IDA]
- histone demethylase activity (H3-K36 specific) [IDA]
- histone demethylase activity (H3-K9 specific) [IDA]
- histone demethylase activity (H4-K20 specific) [IDA]
- iron ion binding [IDA]
- methylated histone binding [IDA]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [IDA]
- protein binding [IPI]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
PREY
AMPD2
PCH9, SPG63, RP5-1160K1.5
adenosine monophosphate deaminase 2
GO Process (7)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.766565634 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.766565634, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID