An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Phospholipid-stimulated autophosphorylation activates the G protein-coupled receptor kinase GRK5.

Kunapuli P, Gurevich VV, Benovic JL

G protein-coupled receptor kinases (GRKs) play an important role in mediating agonist-specific desensitization of numerous G protein-coupled receptors. GRK5, a recently identified member of the GRK family, undergoes a rapid phospholipid-stimulated autophosphorylation to a stoichiometry of approximately 2 mol of phosphate/mol of GRK5. The ability of phospholipids to stimulate autophosphorylation is largely blocked by a glutathione S-transferase fusion protein containing ... [more]

J. Biol. Chem. Apr. 08, 1994; 269(14);10209-12 [Pubmed: 8144599]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

GRK5

Gene Ontology Biological Process

Gene Ontology Molecular Function

phospholipid binding [TAS]protein binding [IPI]protein kinase C binding [TAS]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

RHO