Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Ribosomal protein L12 uses a distinct nuclear import pathway mediated by importin 11.

Plafker SM, Macara IG

Ribosome biogenesis requires the nuclear translocation of ribosomal proteins from their site of synthesis in the cytoplasm to the nucleus. Analyses of the import mechanisms have revealed that most ribosomal proteins can be delivered to the nucleus by multiple transport receptors (karyopherins or importins). We now provide evidence that ribosomal protein L12 (rpL12) is distinguished from the bulk of ribosomal ... [more]

Mol. Cell. Biol. Feb. 01, 2002; 22(4);1266-75 [Pubmed: 11809816]

Throughput

  • Low Throughput

Curated By

  • BioGRID