BAIT

SF3A2

PRP11, PRPF11, SAP62, SF3a66
splicing factor 3a, subunit 2, 66kDa
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein.

Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Luehrmann R

Mass spectrometry was used to identify novel proteins associated with the human 17S U2 snRNP and one of its stable subunits, SF3b. Several additional proteins were identified, demonstrating that 17S U2 snRNPs are significantly more complex than previously thought. Two of the newly identified proteins, namely the DEAD-box proteins SF3b125 and hPrp5 (a homologue of Saccharomyces cerevisiae Prp5p) were characterized ... [more]

EMBO J. Sep. 16, 2002; 21(18);4978-88 [Pubmed: 12234937]

Throughput

  • Low Throughput

Curated By

  • BioGRID