BAIT

IL6R

CD126, IL-6R-1, IL-6RA, IL6Q, IL6RA, IL6RQ, gp80
interleukin 6 receptor
GO Process (25)
GO Function (8)
GO Component (6)
Homo sapiens
PREY

STAT3

ADMIO, APRF, HIES
signal transducer and activator of transcription 3 (acute-phase response factor)
GO Process (27)
GO Function (10)
GO Component (5)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The coiled-coil domain of Stat3 is essential for its SH2 domain-mediated receptor binding and subsequent activation induced by epidermal growth factor and interleukin-6.

Zhang T, Kee WH, Seow KT, Fung W, Cao X

STAT proteins are a family of latent transcription factors that mediate the response to various cytokines and growth factors. Upon stimulation by cytokines, STAT proteins are recruited to the receptors via their SH2 domains, phosphorylated on a specific tyrosine, dimerized, and translocated into the nucleus, where they bind specific DNA sequences and activate the target gene transcription. STATs share highly ... [more]

Mol. Cell. Biol. Oct. 01, 2000; 20(19);7132-9 [Pubmed: 10982829]

Throughput

  • Low Throughput

Curated By

  • BioGRID