BAIT
FGA
Fib2
fibrinogen alpha chain
GO Process (20)
GO Function (4)
GO Component (11)
Gene Ontology Biological Process
- blood coagulation [TAS]
- blood coagulation, common pathway [IMP]
- cell-matrix adhesion [IDA]
- cellular protein complex assembly [IDA]
- extracellular matrix organization [TAS]
- negative regulation of blood coagulation, common pathway [TAS]
- negative regulation of endothelial cell apoptotic process [IDA]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [IDA]
- platelet activation [TAS]
- platelet aggregation [IDA, IMP]
- platelet degranulation [TAS]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of exocytosis [IDA]
- positive regulation of heterotypic cell-cell adhesion [IDA]
- positive regulation of peptide hormone secretion [IDA]
- positive regulation of protein secretion [IDA]
- positive regulation of substrate adhesion-dependent cell spreading [NAS]
- positive regulation of vasoconstriction [IDA]
- protein polymerization [IDA]
- response to calcium ion [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
YWHAQ
14-3-3, 1C5, HS1
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
GO Process (6)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.99999598 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.99999598, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID