Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Identification of an actin binding region and a protein kinase C phosphorylation site on human fascin.

Ono S, Yamakita Y, Yamashiro S, Matsudaira PT, Gnarra JR, Obinata T, Matsumura F

Fascin is a 55-58-kDa actin-bundling protein, the actin binding of which is regulated by phosphorylation (Yamakita, Y., Ono, S., Matsumura, F., and Yamashiro, S. (1996) J. Biol. Chem. 271, 12632-12638). To understand the mechanism of fascin-actin interactions, we dissected the actin binding region and its regulatory site by phosphorylation of human fascin. First, we found that the C-terminal half constitutes ... [more]

J. Biol. Chem. Jan. 24, 1997; 272(4);2527-33 [Pubmed: 8999969]

Throughput

  • Low Throughput

Curated By

  • BioGRID