Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Dual mechanisms for repression of the monomeric orphan receptor liver receptor homologous protein-1 by the orphan small heterodimer partner.

Lee YK, Moore DD

The orphan nuclear hormone receptor liver receptor homologous protein-1 (LRH-1; NR5A2, also known as FTF), an unusual receptor that binds DNA as a monomer, is an essential regulator of expression of a rate-limiting enzyme in bile acid formation, cholesterol 7-alpha-hydroxylase. In a classic negative feedback loop that is a crucial component of the complex regulation of cholesterol metabolism, cholesterol 7-alpha-hydroxylase ... [more]

J. Biol. Chem. Jan. 25, 2002; 277(4);2463-7 [Pubmed: 11668176]

Throughput

  • Low Throughput

Additional Notes

  • mammalian two-hybrid, SHP competes with SRC-3 for interaction with LRH-1 in vivo

Curated By

  • BioGRID