BAIT
ZC2HC1C
C14orf140, FAM164C
zinc finger, C2HC-type containing 1C
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY
PPP1R9B
PPP1R6, PPP1R9, SPINO, Spn
protein phosphatase 1, regulatory subunit 9B
GO Process (11)
GO Function (3)
GO Component (7)
Gene Ontology Biological Process
- RNA splicing [NAS]
- cell cycle arrest [TAS]
- cell migration [IMP]
- cellular response to morphine [ISS]
- filopodium assembly [IMP]
- negative regulation of catalytic activity [NAS]
- negative regulation of cell growth [IDA]
- regulation of cell growth by extracellular stimulus [TAS]
- regulation of cell proliferation [NAS]
- regulation of exit from mitosis [NAS]
- regulation of opioid receptor signaling pathway [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.890445554 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.890445554, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID