NUMB
Gene Ontology Biological Process
- adherens junction organization [IGI]
- axonogenesis [IDA, IMP]
- forebrain development [IMP]
- lateral ventricle development [IMP]
- lung epithelial cell differentiation [IMP]
- negative regulation of Notch signaling pathway [IMP]
- negative regulation of protein localization to plasma membrane [ISO]
- nervous system development [IMP]
- neuroblast division in subventricular zone [IMP]
- neuroblast proliferation [IGI]
- positive regulation of cell migration [ISO]
- positive regulation of dendrite morphogenesis [ISO]
- positive regulation of neurogenesis [IMP]
- positive regulation of polarized epithelial cell differentiation [IMP]
- regulation of neuron differentiation [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EPS15
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Binding specificity and in vivo targets of the EH domain, a novel protein-protein interaction module.
EH is a recently identified protein-protein interaction domain found in the signal transducers Eps15 and Eps15R and several other proteins of yeast nematode. We show that EH domains from Eps15 and Eps15R bind in vitro to peptides containing an asparagine-proline-phenylalanine (NPF) motif. Direct screening of expression libraries with EH domains yielded a number of putative EH interactors, all of which ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID