PXN
Gene Ontology Biological Process
- cell adhesion [NAS]
- cell junction assembly [TAS]
- cellular response to reactive oxygen species [IEP]
- epidermal growth factor receptor signaling pathway [TAS]
- growth hormone receptor signaling pathway [IDA]
- muscle contraction [TAS]
- signal complex assembly [TAS]
- signal transduction [TAS]
- transforming growth factor beta receptor signaling pathway [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NEDD9
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Cytoskeleton-dependent tyrosine phosphorylation of the p130(Cas) family member HEF1 downstream of the G protein-coupled calcitonin receptor. Calcitonin induces the association of HEF1, paxillin, and focal adhesion kinase.
HEF1 is a recently described p130(Cas)-like docking protein that contains one SH3 domain and multiple SH2 binding motifs. In B cells, HEF1 is phosphorylated by a cytoskeleton-dependent mechanism that is triggered by integrin ligation. However, the induction of HEF1 phosphorylation by G protein-coupled receptors has not been reported. We found that HEF1, but not p130(Cas), is tyrosine-phosphorylated following stimulation of ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID