BAIT
ASCL1
ASH1, HASH1, MASH1, bHLHa46
achaete-scute family bHLH transcription factor 1
GO Process (17)
GO Function (8)
GO Component (1)
Gene Ontology Biological Process
- Notch signaling pathway [IDA]
- cerebral cortex GABAergic interneuron differentiation [IEP]
- lung epithelial cell differentiation [NAS]
- negative regulation of apoptotic process [IMP]
- negative regulation of neuron differentiation [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- neurogenesis [IDA]
- noradrenergic neuron development [ISS]
- noradrenergic neuron fate commitment [IMP]
- positive regulation of neuron differentiation [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of gene expression [ISS]
- response to retinoic acid [IEP]
- sympathetic ganglion development [ISS]
- sympathetic nervous system development [NAS]
- ventral spinal cord interneuron fate commitment [ISS]
Gene Ontology Molecular Function- DNA binding [ISS]
- E-box binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- bHLH transcription factor binding [IPI]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding transcription factor activity [ISS]
- DNA binding [ISS]
- E-box binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- bHLH transcription factor binding [IPI]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding transcription factor activity [ISS]
Homo sapiens
PREY
EPPK1
EPIPL, EPIPL1
epiplakin 1
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.946660031 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.946660031, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID