PREY

CIDEB

cell death-inducing DFFA-like effector b
GO Process (4)
GO Function (2)
GO Component (2)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Mitochondria localization and dimerization are required for CIDE-B to induce apoptosis.

Chen Z, Guo K, Toh SY, Zhou Z, Li P

Cell death-inducing DFF45-like effector (CIDE)-B is a member of the novel family of apoptosis-inducing factors that share homology with the N-terminal region of DFF, the DNA fragmentation factor. The molecular mechanism of CIDE-B-induced apoptosis is unclear. We have shown here that CIDE-B protein is localized in mitochondria and forms homodimers and heterodimers with other family members. Serial deletion analyses suggest ... [more]

J. Biol. Chem. Jul. 28, 2000; 275(30);22619-22 [Pubmed: 10837461]

Throughput

  • Low Throughput

Curated By

  • BioGRID