BAIT
KPNA1
IPOA5, NPI-1, RCH2, SRP1
karyopherin alpha 1 (importin alpha 5)
GO Process (10)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- NLS-bearing protein import into nucleus [TAS]
- apoptotic DNA fragmentation [TAS]
- apoptotic process [TAS]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- cytokine-mediated signaling pathway [TAS]
- intracellular transport of virus [TAS]
- positive regulation of protein import into nucleus [ISS]
- regulation of DNA recombination [TAS]
- viral life cycle [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
SMARCE1
BAF57
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
GO Process (6)
GO Function (9)
GO Component (9)
Gene Ontology Biological Process
Gene Ontology Molecular Function- N-acetyltransferase activity [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- chromatin binding [TAS]
- ligand-dependent nuclear receptor binding [IPI]
- nucleosomal DNA binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- transcription coactivator activity [NAS]
- N-acetyltransferase activity [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- chromatin binding [TAS]
- ligand-dependent nuclear receptor binding [IPI]
- nucleosomal DNA binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- transcription coactivator activity [NAS]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.872419705 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.872419705, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID