BAIT

STX4

STX4A, p35-2
syntaxin 4
GO Process (23)
GO Function (4)
GO Component (18)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Functional and biochemical analysis of the C2 domains of synaptotagmin IV.

Thomas DM, Ferguson GD, Herschman HR, Elferink LA

Synaptotagmins (Syts) are a family of vesicle proteins that have been implicated in both regulated neurosecretion and general membrane trafficking. Calcium-dependent interactions mediated through their C2 domains are proposed to contribute to the mechanism by which Syts trigger calcium-dependent neurotransmitter release. Syt IV is a novel member of the Syt family that is induced by cell depolarization and has a ... [more]

Mol. Biol. Cell Jul. 01, 1999; 10(7);2285-95 [Pubmed: 10397765]

Throughput

  • Low Throughput

Curated By

  • BioGRID