BAIT
GHRL
MTLRP, UNQ524/PRO1066
ghrelin/obestatin prepropeptide
GO Process (36)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISS, TAS]
- actin polymerization or depolymerization [IDA]
- activation of MAPK activity [IMP]
- adult feeding behavior [ISS]
- cartilage development [NAS]
- cellular protein metabolic process [TAS]
- cortisol secretion [NAS]
- decidualization [IDA]
- dendrite development [IDA]
- gastric acid secretion [IBA]
- glucose metabolic process [NAS]
- growth hormone secretion [TAS]
- hormone-mediated signaling pathway [TAS]
- negative regulation of angiogenesis [NAS]
- negative regulation of apoptotic process [IDA]
- negative regulation of circadian sleep/wake cycle, REM sleep [IDA]
- negative regulation of endothelial cell proliferation [IDA]
- negative regulation of inflammatory response [IDA]
- negative regulation of interleukin-1 beta production [IDA]
- negative regulation of interleukin-6 biosynthetic process [IDA]
- negative regulation of tumor necrosis factor biosynthetic process [IDA]
- positive regulation of appetite [ISS]
- positive regulation of circadian sleep/wake cycle, non-REM sleep [IDA]
- positive regulation of corticotropin secretion [IDA]
- positive regulation of cortisol secretion [IDA]
- positive regulation of cytosolic calcium ion concentration [ISS]
- positive regulation of growth hormone receptor signaling pathway [IC]
- positive regulation of growth hormone secretion [IDA]
- positive regulation of insulin secretion [ISS]
- positive regulation of multicellular organism growth [IC]
- positive regulation of protein tyrosine kinase activity [IMP]
- positive regulation of synapse assembly [IDA]
- regulation of cell proliferation [IDA]
- regulation of response to food [IBA]
- response to estrogen [IDA]
- response to hormone [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SIRT5
SIR2L5
sirtuin 5
GO Process (7)
GO Function (5)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.894031336 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.894031336, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID