STX17
Gene Ontology Biological Process
- ER to Golgi vesicle-mediated transport [IMP]
- Golgi organization [IMP]
- autophagic vacuole fusion [IMP]
- endoplasmic reticulum-Golgi intermediate compartment organization [IMP]
- intracellular protein transport [IBA]
- protein localization to pre-autophagosomal structure [IDA]
- vesicle docking [IBA]
- vesicle fusion [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- ER to Golgi transport vesicle [ISS]
- ER-mitochondrion membrane contact site [IDA]
- SNARE complex [IDA]
- autophagic vacuole membrane [IDA]
- cytosol [ISS]
- endoplasmic reticulum membrane [IDA]
- endoplasmic reticulum-Golgi intermediate compartment [IDA]
- integral component of membrane [IBA]
- lysosomal membrane [IDA]
- mitochondrion [IDA]
- rough endoplasmic reticulum [IDA]
- smooth endoplasmic reticulum membrane [ISS]
SNAP29
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Three novel proteins of the syntaxin/SNAP-25 family.
Intracellular membrane traffic is thought to be regulated in part by soluble N-ethylmaleimide-sensitive factor-attachment protein receptors (SNAREs) through the formation of complexes between these proteins present on vesicle and target membranes. All known SNARE-mediated fusion events involve members of the syntaxin and vesicle-associated membrane protein families. The diversity of mammalian membrane compartments predicts the existence of a large number of ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SNAP29 STX17 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3182794 | |
| STX17 SNAP29 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SNAP29 STX17 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID