Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Interaction of cytochrome c with cytochrome bc1 complex of the mitochondrial respiratory chain.

Broger C, Nalecz MJ, Azzi A

The binding of cytochrome c to the cytochrome bc1 complex of bovine heart mitochondria was studied. Cytochrome c derivatives, arylazido-labeled at lysine 13 or lysine 22, were prepared and their properties as electron acceptors from the bc1 complex were measured. Mixtures of bc1 complex with cytochrome c derivatives were illuminated with ultraviolet light and afterwards subjected to polyacrylamide gel electrophoresis. ... [more]

Biochim. Biophys. Acta Oct. 03, 1980; 592(3);519-27 [Pubmed: 6251869]

Throughput

  • Low Throughput

Curated By

  • BioGRID