PKP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DSP
Gene Ontology Biological Process
- apoptotic process [TAS]
- bundle of His cell to Purkinje myocyte communication [IMP]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- desmosome organization [ISS]
- epidermis development [TAS]
- intermediate filament organization [ISS]
- keratinocyte differentiation [IDA]
- peptide cross-linking [IDA]
- protein localization to adherens junction [ISS]
- regulation of heart rate by cardiac conduction [IMP]
- ventricular cardiac muscle cell action potential [IMP]
- ventricular compact myocardium morphogenesis [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Interaction of plakophilins with desmoplakin and intermediate filament proteins: an in vitro analysis.
Plakophilin 1 and 2 (PKP1, PKP2) are members of the arm-repeat protein family. They are both constitutively expressed in most vertebrate cells, in two splice forms named a and b, and display a remarkable dual location: they occur in the nuclei of cells and, in epithelial cells, at the plasma membrane within the desmosomal plaques. We have shown by solid ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PKP1 DSP | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 12.8488 | BioGRID | 2950543 | |
| PKP1 DSP | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID