Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Cabin 1, a negative regulator for calcineurin signaling in T lymphocytes.

Sun L, Youn HD, Loh C, Stolow M, He W, Liu JO

Calcineurin plays a pivotal role in the T cell receptor (TCR)-mediated signal transduction pathway and serves as a common target for the immunosuppressants FK506 and cyclosporin A. We report the identification of a novel endogenous calcineurin binding protein named Cabin 1 that inhibits calcineurin-mediated signal transduction. The interaction between Cabin 1 and calcineurin is dependent on PKC activation. Overexpression of ... [more]

Immunity Jun. 01, 1998; 8(6);703-11 [Pubmed: 9655484]

Throughput

  • Low Throughput

Curated By

  • BioGRID