BAIT

YWHAE

14-3-3E, HEL2, KCIP-1, MDCR, MDS
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
Homo sapiens
PREY

TOP2A

TOP2, TP2A
topoisomerase (DNA) II alpha 170kDa
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Modulation of human DNA topoisomerase IIalpha function by interaction with 14-3-3epsilon.

Kurz EU, Leader KB, Kroll DJ, Clark M, Gieseler F

Human DNA topoisomerase IIalpha (topo II), a ubiquitous nuclear enzyme, is essential for normal and neoplastic cellular proliferation and survival. Several common anticancer drugs exert their cytotoxic effects through interaction with topo II. In experimental systems, altered topo II expression has been associated with the appearance of drug resistance. This mechanism, however, does not adequately account for clinical cases of ... [more]

J. Biol. Chem. May. 05, 2000; 275(18);13948-54 [Pubmed: 10788521]

Throughput

  • Low Throughput

Curated By

  • BioGRID