RGS5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GNAI1
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISS]
- adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway [TAS]
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [IBA]
- blood coagulation [TAS]
- cell division [IMP]
- platelet activation [TAS]
- response to peptide hormone [ISS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Characterization of RGS5 in regulation of G protein-coupled receptor signaling.
RGS proteins (regulators of G protein signaling) serve as GTPase-activating proteins (GAPs) for G alpha subunits and negatively regulate G protein-coupled receptor signaling. In this study, we characterized biochemical properties of RGS5 and its N terminal (1-33)-deleted mutant (deltaN-RGS5). RGS5 bound to G alpha(i1), G alpha(i2), G alpha(i3), G alpha(o) and G alpha(q) but not to G alpha(s) and G ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID