An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Characterisation of alpha-dystrobrevin in muscle.

Nawrotzki R, Loh NY, Ruegg MA, Davies KE, Blake DJ

Dystrophin-related and associated proteins are important for the formation and maintenance of the mammalian neuromuscular junction. Initial studies in the electric organ of Torpedo californica showed that the dystrophin-related protein dystrobrevin (87K) co-purifies with the acetylcholine receptors and other postsynaptic proteins. Dystrobrevin is also a major phosphotyrosine-containing protein in the postsynaptic membrane. Since inhibitors of tyrosine protein phosphorylation block acetylcholine ... [more]

J. Cell. Sci. Sep. 01, 1998; 111(0);2595-605 [Pubmed: 9701558]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UTRN DTNA	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cho NH (2022)	High	-	BioGRID	3374574
UTRN DTNA	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Marcon E (2014)	High	36.256	BioGRID	2943608
UTRN DTNA	Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe.	Tommasi di Vignano A (2000)	Low	-	BioGRID	-
DTNA UTRN	Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe.	Tommasi di Vignano A (2000)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

UTRN

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin binding [IDA]integrin binding [IPI]protein binding [IPI]protein kinase binding [IPI]vinculin binding [IPI]

Gene Ontology Cellular Component

DTNA