BAIT
CHRNA4
BFNC, EBN, EBN1, NACHR, NACHRA4, NACRA4
cholinergic receptor, nicotinic, alpha 4 (neuronal)
GO Process (21)
GO Function (4)
GO Component (7)
Gene Ontology Biological Process
- B cell activation [ISS]
- DNA repair [IMP]
- action potential [ISS]
- behavioral response to nicotine [IMP]
- calcium ion transport [ISS]
- cation transmembrane transport [IDA, TAS]
- cognition [IMP]
- ion transmembrane transport [TAS]
- ion transport [NAS]
- membrane depolarization [ISS]
- neurological system process [IMP]
- regulation of dopamine secretion [ISS]
- regulation of inhibitory postsynaptic membrane potential [ISS]
- regulation of membrane potential [ISS]
- response to hypoxia [IDA]
- response to nicotine [IDA]
- response to oxidative stress [IMP]
- sensory perception of pain [ISS]
- signal transduction [IDA]
- synaptic transmission [NAS, TAS]
- synaptic transmission, cholinergic [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PPAP2A
LLP1a, LPP1, PAP-2a, PAP2
phosphatidic acid phosphatase type 2A
GO Process (11)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
- androgen receptor signaling pathway [NAS]
- germ cell migration [TAS]
- intracellular steroid hormone receptor signaling pathway [TAS]
- lipid metabolic process [NAS]
- negative regulation of cell proliferation [NAS]
- phospholipid dephosphorylation [TAS]
- protein kinase C-activating G-protein coupled receptor signaling pathway [TAS]
- regulation of lipid metabolic process [NAS]
- small molecule metabolic process [TAS]
- sphingolipid biosynthetic process [TAS]
- sphingolipid metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.802202035 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.802202035, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID