BAIT
KCTD12
C13orf2, PFET1, PFETIN
potassium channel tetramerization domain containing 12
GO Process (0)
GO Function (1)
GO Component (1)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
NUDT16
nudix (nucleoside diphosphate linked moiety X)-type motif 16
GO Process (14)
GO Function (15)
GO Component (4)
Gene Ontology Biological Process
- IDP catabolic process [IDA]
- XDP catabolic process [IDA]
- adenosine to inosine editing [IMP]
- dITP catabolic process [ISS]
- mRNA catabolic process [IDA]
- negative regulation of rRNA processing [ISS]
- nucleobase-containing small molecule catabolic process [TAS]
- nucleobase-containing small molecule metabolic process [TAS]
- positive regulation of cell cycle process [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of double-strand break repair [IMP]
- proteolysis [IDA]
- small molecule metabolic process [TAS]
- snoRNA catabolic process [IDA]
Gene Ontology Molecular Function- GTP binding [IDA]
- ITP binding [IDA]
- XTP binding [IDA]
- cobalt ion binding [IDA]
- dIDP diphosphatase activity [EXP]
- dITP diphosphatase activity [ISS]
- inosine-diphosphatase activity [EXP]
- m7G(5')pppN diphosphatase activity [IDA]
- mRNA binding [IDA]
- magnesium ion binding [IDA]
- manganese ion binding [IDA]
- metalloexopeptidase activity [IDA]
- nucleotide phosphatase activity, acting on free nucleotides [IDA]
- protein homodimerization activity [IDA]
- snoRNA binding [IDA]
- GTP binding [IDA]
- ITP binding [IDA]
- XTP binding [IDA]
- cobalt ion binding [IDA]
- dIDP diphosphatase activity [EXP]
- dITP diphosphatase activity [ISS]
- inosine-diphosphatase activity [EXP]
- m7G(5')pppN diphosphatase activity [IDA]
- mRNA binding [IDA]
- magnesium ion binding [IDA]
- manganese ion binding [IDA]
- metalloexopeptidase activity [IDA]
- nucleotide phosphatase activity, acting on free nucleotides [IDA]
- protein homodimerization activity [IDA]
- snoRNA binding [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.983754442 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.983754442, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID