BAIT
S100A8
60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8
S100 calcium binding protein A8
GO Process (16)
GO Function (7)
GO Component (7)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- autophagy [IDA]
- chemokine production [TAS]
- cytokine production [TAS]
- defense response to bacterium [TAS]
- defense response to fungus [TAS]
- inflammatory response [TAS]
- leukocyte migration involved in inflammatory response [IDA]
- neutrophil aggregation [IDA]
- neutrophil chemotaxis [IDA]
- positive regulation of NF-kappaB transcription factor activity [TAS]
- positive regulation of cell growth [TAS]
- positive regulation of inflammatory response [IDA]
- positive regulation of intrinsic apoptotic signaling pathway [IDA]
- regulation of cytoskeleton organization [TAS]
- sequestering of zinc ion [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GOT1
ASTQTL1, GIG18, cAspAT, cCAT
glutamic-oxaloacetic transaminase 1, soluble
GO Process (16)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
- 2-oxoglutarate metabolic process [ISS]
- L-methionine biosynthetic process from methylthioadenosine [TAS]
- aspartate catabolic process [IDA]
- aspartate metabolic process [ISS]
- carbohydrate metabolic process [TAS]
- cellular amino acid biosynthetic process [TAS]
- cellular nitrogen compound metabolic process [TAS]
- cellular response to insulin stimulus [IEP]
- gluconeogenesis [TAS]
- glucose metabolic process [TAS]
- glutamate metabolic process [ISS]
- glycerol biosynthetic process [ISS]
- polyamine metabolic process [TAS]
- response to glucocorticoid [IEP]
- small molecule metabolic process [TAS]
- sulfur amino acid metabolic process [TAS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.905498942 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.905498942, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID