BAIT
SYT2
MYSPC, SytII
synaptotagmin II
GO Process (2)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
VLDLR
CARMQ1, CHRMQ1, VLDLRCH, RP11-320E16.1
very low density lipoprotein receptor
GO Process (9)
GO Function (9)
GO Component (3)
Gene Ontology Biological Process
- glycoprotein transport [IDA]
- memory [TAS]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- nervous system development [TAS]
- positive regulation of dendrite development [ISS]
- receptor-mediated endocytosis [IDA]
- reelin-mediated signaling pathway [ISS]
- signal transduction [TAS]
- very-low-density lipoprotein particle clearance [IDA]
Gene Ontology Molecular Function- apolipoprotein binding [IDA, ISS]
- calcium-dependent protein binding [IPI]
- glycoprotein binding [IPI]
- glycoprotein transporter activity [IDA]
- low-density lipoprotein receptor activity [TAS]
- protein binding [IPI]
- reelin receptor activity [ISS]
- very-low-density lipoprotein particle binding [IDA]
- very-low-density lipoprotein particle receptor activity [IDA]
- apolipoprotein binding [IDA, ISS]
- calcium-dependent protein binding [IPI]
- glycoprotein binding [IPI]
- glycoprotein transporter activity [IDA]
- low-density lipoprotein receptor activity [TAS]
- protein binding [IPI]
- reelin receptor activity [ISS]
- very-low-density lipoprotein particle binding [IDA]
- very-low-density lipoprotein particle receptor activity [IDA]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.999999911 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.999999911, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID